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KMID : 0368420070500040403
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Journal of Plant Biology
2007 Volume.50 No. 4 p.403 ~ p.403
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Construction and profiling of a cDNA library from young fruit of satsuma mandarin
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Boo Kyung-Hwan
Kim Dae-Woon
Cho Somi-Kim
Jin Seong-Beom
Kim Jae-Hoon
Lee Hyo-Yeon
Riu Key-Zung
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Abstract
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To profile gene expression in the early stage of fruit development from ‘Nichinan No. 1’ satsuma mandarin (Citrus unshiu Marc.), we isolated total mRNA at 30 d after flowering. A cDNA library was prepared from mature mRNAs and a total of 2350 cDNA clones were partially sequenced. In all, 1914 ESTs were acquired after the removal of the vector sequence and filtering over a minimum length of 150 nucleotides. A total of 763 unigenes, consisting of 138 contigs and 625 singletons, was identified after assembly of those ESTs. According to our homology search with BLASTX against the NCBI database, the deduced amino acid sequences of 253 unigenes were homologous to proteins with known function and 242 unigenes were significantly matched to proteins with putative or unknown functions. The remaining 268 showed no significant similarity to any protein sequences found in the public database with matches higher than an E value of 10-5. The 253 unigenes matched to proteins with known function were then manually assigned to 10 cellular functional categories using a modified MIPS MATDB classification. The expression level of each gene was analyzed based on the redundancy of cDNA clones in each contig that comprised more than 10 ESTs. Here, the most abundant gene expressed in young fruits was for a chitinase precursor. A miraculin-like protein and a lectin-related protein precursor were also abundant.
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KEYWORD
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cDNA library, Citrus, ESTs, gene, young fruit
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